Npgrj_nsmb_1314 1127..1133
نویسندگان
چکیده
The anticancer drug cisplatin forms 1,2-d(GpG) DNA intrastrand cross-links (cisplatin lesions) that stall RNA polymerase II (Pol II) and trigger transcription-coupled DNA repair. Here we present a structure-function analysis of Pol II stalling at a cisplatin lesion in the DNA template. Pol II stalling results from a translocation barrier that prevents delivery of the lesion to the active site. AMP misincorporation occurs at the barrier and also at an abasic site, suggesting that it arises from nontemplated synthesis according to an ‘A-rule’ known for DNA polymerases. Pol II can bypass a cisplatin lesion that is artificially placed beyond the translocation barrier, even in the presence of a G.A mismatch. Thus, the barrier prevents transcriptional mutagenesis. The stalling mechanism differs from that of Pol II stalling at a photolesion, which involves delivery of the lesion to the active site and lesion-templated misincorporation that blocks transcription.
منابع مشابه
Nucleotide sequence of the Saccharomyces cerevisiae CLN1 and CLN2 genes
The Saccharomyces cerevisiae CLNJ and CLN2 genes were S.I.R. from the National Institute of General Medical Sciences. isolated as high-dosage suppressors of a temperature-sensitive S.I.R. also acknowledges support from American Cancer Society mutation of the CDC28 gene, which encodes a protein kinase Faculty Research Award FRA-248. required for cell division. The CLNJ and CLN2 genes encode prot...
متن کاملA study of Epistemic Modality in Academic and Popularised Discourse: the case of Possibility Adverbs Perhaps, Maybe and Possibly*
This paper describes the use of perhaps, maybe and possibly in a crossdisciplinary corpus of academic and popularised scientific writing. It accounts for their higher frequency in popularised discourse by investigating their functions in detail. The analysis, conducted from various perspectives (syntactic, semantic, pragmatic and rhetorical), suggests that two factors are at work: the evidentia...
متن کاملA Possible Design of the Linac-to-LEB Injection Girder for 1133 MeV H− Ions
We present a design of the girder to accommodate injection of 1133 MeV kinetic energy H− ions from the linac [1] into the LEB. This design is based on Colton and Thiessen’s design [2] for 600 MeV ions, and does not require a modification of the current [3] LEB lattice.
متن کاملLibert , Food - Derived Odors Life Span by Olfaction andDrosophilaRegulation
http://www.sciencemag.org/cgi/content/full/315/5815/1133 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/1136610/DC1 can be found at: Supporting Online Material found at: can be related to this article A list of selected additional articles on the Science Web sites http://www.s...
متن کامل